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PE anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2H7_PE_021805
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE (solid line), or mouse IgG2b, κ PE (dashed line).
  • 2H7_PE_021805
    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE (solid line), or mouse IgG2b, κ PE (dashed line).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
302305 25 tests £50
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302306 100 tests £102
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302346 100 µg £142
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  2. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  3. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  4. Kisu I, et al. 2019. J Clin Med. 1.425. PubMed
  5. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  6. Chowdhury A, et al. 2015. J Immunol. 195: 3237 - 3247. PubMed
  7. Weinstein S, et al. 2016. Proc Natl Acad Sci U S A. 113: 16 - 22. PubMed
  8. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  9. Schreibelt G, et al. 2016. Clin Cancer Res. 22: 2155 - 2166. PubMed
  10. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
  11. Alfaro C, et al. 2016. Clin Cancer Res. 22: 3924 - 3936. PubMed
  12. Roider T, et al. 2021. Blood Adv. 5:5060. PubMed
  13. Wood MP, et al. 2020. J Infect Dis. 222:44. PubMed
  14. Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed
  15. Pekayvaz K, et al. 2022. Nat Commun. 13:1018. PubMed
  16. Keim D, et al. 2021. Int J Mol Sci. 22:. PubMed
  17. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  18. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  19. Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed
  20. Wang P, et al. 2023. Front Immunol. 13:1104329. PubMed
  21. Sy M, et al. 2023. J Neuroinflammation. 209:20. PubMed
RRID
AB_314253 (BioLegend Cat. No. 302305)
AB_314254 (BioLegend Cat. No. 302306)
AB_2564144 (BioLegend Cat. No. 302346)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Go To Top Version: 3    Revision Date: 10/08/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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