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PE anti-human CD223 (LAG-3) Antibody

Pricing & Availability
Clone
11C3C65 (See other available formats)
Regulatory Status
RUO
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
11C3C65_PE_CD223_Antibody_1_080916
CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with CD8 FITC and CD223 (clone 11C3C65) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 11C3C65_PE_CD223_Antibody_1_080916
    CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with CD8 FITC and CD223 (clone 11C3C65) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 11C3C65_PE_CD223_Antibody_2_080916
See PE spectral data
Cat # Size Price Quantity Check Availability Save
369305 25 tests £97
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369306 100 tests £247
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Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LAG-3 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.

Product Citations
  1. Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
  2. Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed
  3. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  4. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  5. Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed
  6. Edwards CJ, et al. 2021. Br J Cancer. . PubMed
  7. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
  8. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  9. Arasanz H, et al. 2022. Cancers (Basel). 14: . PubMed
  10. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  11. Imai H, et al. 2023. iScience. 26:106822. PubMed
RRID
AB_2629591 (BioLegend Cat. No. 369305)
AB_2629592 (BioLegend Cat. No. 369306)

Antigen Details

Structure
70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution

Activated T-cells and natural killer cells (NKs) and regulatory T cells.

Function
Negatively regulates T-cell activation.
Ligand/Receptor
Binds MHC class II molecules.
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.

Gene ID
3902 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/12/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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