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- Clone
- p282 (H19) (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V S006
- Other Names
- Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes stained with p282 PE -
Pre-lysed human blood leukocytes were stained with CD59 (clone P282) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 304707 | 25 tests | £71 | ||||
| 304708 | 100 tests | £163 | ||||
CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.
Product DetailsProduct Details
- Reactivity
- Human,Cynomolgus,Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Product Citations
- RRID
-
AB_2275871 (BioLegend Cat. No. 304707)
AB_2076125 (BioLegend Cat. No. 304708)
Antigen Details
- Structure
- Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
- Distribution
-
All cell types
- Function
- Blocks complement-mediated lysis by inhibiting MAC formation
- Ligand/Receptor
- Complement components C8, C9
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Davies A, et al. 1993. Immunol. Res. 12:258.
2. Lachmann P. 1991. Immunol. Today 12:312.
3. Liszewski M, et al. 1996. Adv. Immunol. 61:201. - Gene ID
- 966 View all products for this Gene ID
- UniProt
- View information about CD59 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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