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PE anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK1_PE_112409
Human peripheral blood lymphocytes stained with SK1 PE.
  • SK1_PE_112409
    Human peripheral blood lymphocytes stained with SK1 PE.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
344705 25 tests £91
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344706 100 tests £223
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Meng W,et al. 2017. Antimicrob Agents Chemother.. 10.1128/AAC.01719-17. PubMed
  2. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  3. Wakeley ME, et al. 2020. J Surg Res. 245:610. PubMed
  4. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  5. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  6. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  7. You L, et al. 2020. Front Immunol. 1.699305556. PubMed
  8. Della–Torre E, et al. 2018. Arthritis Rheumatol. 70:1133. PubMed
  9. Price A, et al. 2016. Proc Natl Acad Sci U S A. 113: 10678 - 1068. PubMed
  10. Gimenez-Rivera V, et al. 2016. J Immunol. 197(11):4240-4246. PubMed
  11. Chen J, et al. 2021. J Invest Dermatol. 787:141. PubMed
  12. Vandooren J, et al. 2021. Front Immunol. 12:701739. PubMed
  13. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  14. Zhang H, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  15. Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
  16. Landry LG, et al. 2021. Bio Protoc. 11:e3883. PubMed
  17. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  18. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  19. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
  20. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  21. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  22. Lee IK, et al. 2020. Cancers (Basel). 12:. PubMed
  23. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  24. Wang N, et al. 2021. iScience. 24:103205. PubMed
  25. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
  26. Ng CY, et al. 2022. EMBO Rep. 23:e54271. PubMed
  27. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  28. Ng CY, et al. 2022. EMBO Rep. 23:e55871. PubMed
  29. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  30. Amer HT, et al. 2022. Front Mol Biosci. 9:1032517. PubMed
  31. Le Y, et al. 2022. iScience. 25:105426. PubMed
  32. Mohamed H, et al. 2023. Biomedicines. 11: . PubMed
  33. Yang Y, et al. 2023. Cancers (Basel). 15:. PubMed
  34. Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed
  35. Li J, et al. 2023. Sci Rep. 13:7501. PubMed
  36. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
RRID
AB_1953243 (BioLegend Cat. No. 344705)
AB_1953244 (BioLegend Cat. No. 344706)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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