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PE anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_PE_122107
C57BL/6 bone marrow cells stained with TER-119 PE and anti-mouse CD45 APC
  • TER-119_PE_122107
    C57BL/6 bone marrow cells stained with TER-119 PE and anti-mouse CD45 APC
See PE spectral data
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116207 50 µg £50
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116208 200 µg £131
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
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  5. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  6. Wheeler MA, et al. 2019. Cell. 176:581. PubMed
  7. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
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  13. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
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  21. Toda S, et al. 2014. Blood. 123:3963. PubMed
  22. Duek A, et al. 2014. Blood. 123:3943. PubMed
  23. Hsieh W, et al. 2015. Gut. 64:312. PubMed
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  26. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  27. Endo Y, et al. 2020. FASEB J. 34:16086. PubMed
  28. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  29. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  30. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  31. Sun D, et al. 2021. Cell Stem Cell. . PubMed
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  34. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  35. Wheeler MA, et al. 2020. Nature. 578:593. PubMed
  36. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  37. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  38. Jia W, et al. 2020. Theranostics. 10:6886. PubMed
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RRID
AB_313708 (BioLegend Cat. No. 116207)
AB_313709 (BioLegend Cat. No. 116208)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/04/2013

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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