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PE/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_PECy7_060507
Human peripheral blood lymphocytes stained with OKT4 PE/Cyanine7
  • OKT4_PECy7_060507
    Human peripheral blood lymphocytes stained with OKT4 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
317413 25 tests £105
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317414 100 tests £213
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Ellis-Connell AL, et al. 2018. J Virol. 92:e01748. PubMed
  2. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  3. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
  4. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  5. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  6. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  7. Spitsin S, et al. 2020. Mol Ther Methods Clin Dev. 17:1088. PubMed
  8. Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed
  9. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  10. Della–Torre E, et al. 2018. Arthritis Rheumatol. 70:1133. PubMed
  11. Blom L, et al. 2011. PLoS One. 6:e21695. PubMed
  12. Soriano-Sarabia N, et al. 2012. J Immunol. 189:5212. PubMed
  13. Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed
  14. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  15. Lieberman L, et al. 2016. Neurology. 86: 375 - 381. PubMed
  16. Coiras M, et al. 2016. Cell Rep. 14:2100-2107. PubMed
  17. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  18. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  19. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  20. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  21. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  22. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  23. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  24. Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
  25. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  26. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  27. Podaza E, et al. 2020. Front Immunol. 11:1147. PubMed
  28. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  29. Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
  30. Ataya M, et al. 2020. Am J Transplant. 20:663. PubMed
  31. Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed
  32. Kwee BJ, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  33. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  34. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  35. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  36. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  37. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  38. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  39. Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed
RRID
AB_571958 (BioLegend Cat. No. 317413)
AB_571959 (BioLegend Cat. No. 317414)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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