- Clone
- JES10-5A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-13, NC30
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![JES10-5A2_PECyanine7_IL-13_Antibody_1_112023 JES10-5A2_PECyanine7_IL-13_Antibody_1_112023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/JES10-5A2_PECyanine7_IL-13_Antibody_1_112023.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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TH2 polarized human peripheral blood lymphocytes were surface stained with PE anti-human CD3 then intracellularly stained with IL-13 (clone JES10-5A2) PE/Cyanine7 (left) or rat IgG1, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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501913 | 25 tests | £121 | ||||
501914 | 100 tests | £262 |
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-expressed, recombinant human IL-13
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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ELISA or ELISPOT Capture1,2: The Ultra-LEAF™ Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
- Additional Product Notes
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BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
- Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
- Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
- Andersson, J., et al. 1994. Immunology 83:16.
- Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
- Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
- Product Citations
- RRID
-
AB_2616745 (BioLegend Cat. No. 501913)
AB_2616746 (BioLegend Cat. No. 501914)
Antigen Details
- Structure
- Cytokine; 17 kD (Mammalian)
- Bioactivity
- Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
- Cell Sources
- Activated T cells, mast cells, NK cells
- Cell Targets
- B cells, monocytes, macrophages, basophils
- Receptors
- Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028. - Regulation
- IL-13Rα2 possible antagonist
- Gene ID
- 3596 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
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