- Clone
- 581 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V MA27
- Other Names
- Gp105-120, My10
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![581_PEDZL594_CD34_Antibody_FC_1_071614.jpg 581_PEDZL594_CD34_Antibody_FC_1_071614.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/581_PEDZL594_CD34_Antibody_FC_1_071614.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood leukocytes were stained with CD14 Brilliant Violet 421™, CD45 APC, and CD34 (clone 581) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom). Cells were gated on total CD14 negative lymphocyte population. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
343533 | 25 tests | £97 | ||||
343534 | 100 tests | £241 |
CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
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Application References
(PubMed link indicates BioLegend citation) -
- Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
- Felschow DM, et al. 2001. Blood 97:3768.
- Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
- Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
- Product Citations
- RRID
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AB_2564011 (BioLegend Cat. No. 343533)
AB_2564012 (BioLegend Cat. No. 343534)
Antigen Details
- Structure
- 105-120 kD single chain mucin-like glycoprotein
- Distribution
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Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
- Function
- Cell adhesion
- Ligand/Receptor
- L-selectin, E-selectin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey. - Gene ID
- 947 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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