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PE Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
  • Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
    C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
406421 100 µg £72
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Adler D, et al. 2019. iScience. 15:39. PubMed
  2. Ramovs V, et al. 2019. Breast Cancer Res. 21:63. PubMed
  3. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  4. Zuidema A, et al. 2018. J Cell Sci. 131. PubMed
  5. Kwok HS, et al. 2019. ACS Chem Biol. 14:603. PubMed
  6. Suzuki K, et al. 2019. Int J Mol Med. 44:1187. PubMed
  7. Komegae EN, et al. 2019. Front Immunol. 10:1496. PubMed
  8. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  9. Thirugnanam K, et al. 2019. J Am Heart Assoc. 8:e012792. PubMed
  10. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  11. Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
  12. Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed
  13. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  14. Claus M, Wingert S, Watzl C 2016. Open Bio. 6: 160010. PubMed
  15. Carmona-Rivera C, et al. 2020. JCI Insight. 5:00. PubMed
  16. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  17. Fujiwara Y, et al. 2020. Clin Transl Immunology. 9:e1162. PubMed
  18. van Nie L, et al. 2020. Sci Rep. 10:11928. PubMed
  19. Kim EH, et al. 2020. Elife. 9:00. PubMed
  20. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  21. Routhu NK, et al. 2021. Immunity. 54(3):542-556.e9. PubMed
  22. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  23. Ramovs V, et al. 2020. J Invest Dermatol. . PubMed
  24. Shukla GS, et al. 2020. Clin Transl Oncol. 22:1094. PubMed
  25. Liang F, et al. 2021. Front Immunol. 12:658715. PubMed
  26. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  27. Routhu NK, et al. 2021. Nat Commun. 12:3587. PubMed
  28. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  29. You C, et al. 2021. Cancers (Basel). 13:. PubMed
  30. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  31. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  32. Zuidema A, et al. 2022. J Cell Sci. 135:. PubMed
  33. Ryniawec JM, et al. 2022. Diagnostics (Basel). 12:. PubMed
  34. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  35. Hsiue EH, et al. 2021. Science. 371:. PubMed
  36. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  37. Kim S, et al. 2021. Elife. 10:. PubMed
  38. Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed
  39. Secchia S, et al. 2022. Dev Cell. 57:496. PubMed
  40. Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed
  41. Eldi P, et al. 2022. Immunol Cell Biol. 100:250. PubMed
  42. Zuidema A, et al. 2022. J Cell Biol. 221: . PubMed
  43. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  44. Zhang H, et al. 2022. Adv Sci (Weinh). :e2204463. PubMed
  45. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  46. Wang Y, et al. 2023. iScience. 26:106630. PubMed
RRID
AB_2563484 (BioLegend Cat. No. 406421)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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