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PerCP anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_PerCP_020110
Human peripheral blood lymphocytes stained with OKT4 PerCP
  • OKT4_PerCP_020110
    Human peripheral blood lymphocytes stained with OKT4 PerCP
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
317431 25 tests £105
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317432 100 tests £230
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Vyas AK, et al. 2019. Hepatol Commun. 3:795. PubMed
  2. Shan L, et al. 2017. Immunity. 47:766. PubMed
  3. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  4. Liu X, et al. 2013. PLoS One. 8:64531. PubMed
  5. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  6. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  7. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  8. Walsh AJ, et al. 2020. Nat Biomed Eng. . PubMed
  9. Zhang H, et al. 2021. Front Immunol. 12:644520. PubMed
  10. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  11. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  12. Turner JS, et al. 2021. Nature. 596:109. PubMed
  13. Buters TP, et al. 2021. Br J Clin Pharmacol. Online ahead of print. PubMed
  14. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  15. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
  16. Nguyen HM, et al. 2020. Mediators Inflamm. 2020:6705428. PubMed
  17. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  18. Li P, et al. 2022. Front Oncol. 12:917400. PubMed
  19. Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed
  20. Braun T, et al. 2023. Front Immunol. 14:1086433. PubMed
RRID
AB_2028492 (BioLegend Cat. No. 317431)
AB_2028494 (BioLegend Cat. No. 317432)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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