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PerCP/Cyanine5.5 anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OKT3_PerCPCy5.5_CD3_Antibody_FC_100312
Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • OKT3_PerCPCy5.5_CD3_Antibody_FC_100312
    Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
317335 25 tests £77
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317336 100 tests £157
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed
  2. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
  3. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  4. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  5. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  6. Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
  7. Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
  8. Wang J, et al. 2020. Cell. 183:1264. PubMed
  9. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  10. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  11. Lee E, et al. 2021. Sci Rep. 11:14841. PubMed
  12. Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed
  13. Bulgarelli J, et al. 2021. Front Immunol. 12:778459. PubMed
  14. Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Kasper M, et al. 2021. Elife. 10:. PubMed
  16. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  17. Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
  18. Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
  19. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  20. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  21. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  22. Alonso R, et al. 2022. Blood Adv. :. PubMed
  23. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  24. Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed
  25. Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed
  26. Khuisangeam N, et al. 2022. J Immunol Res. 2022:2449373. PubMed
  27. Beck C, et al. 2023. Biomedicines. 11:. PubMed
  28. Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed
RRID
AB_2561627 (BioLegend Cat. No. 317335)
AB_2561628 (BioLegend Cat. No. 317336)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 05/24/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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