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Purified anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1_Purified_071007
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with purified 4B4-1, followed by biotinylated anti-mouse IgG and Sav-PE
  • 4B4-1_Purified_071007
    PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with purified 4B4-1, followed by biotinylated anti-mouse IgG and Sav-PE
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309802 100 µg £90
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  2. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  3. Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed
  4. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  5. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  6. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  7. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  8. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  9. Ichikawa J, et al. 2020. Clin Cancer Res. 26:3384. PubMed
  10. Sun R, et al. 2022. Cells. 11:. PubMed
  11. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  12. Tang JS, et al. 2020. Food Funct. 11:5782. PubMed
  13. Law JC, et al. 2021. J Immunol. 206:37. PubMed
  14. Lu X, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_314781 (BioLegend Cat. No. 309802)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 2    Revision Date: 08/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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