- Clone
- BNI3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BNI3_Purified_CD152_Antibody_1_040720 BNI3_Purified_CD152_Antibody_1_040720](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BNI3_Purified_CD152_Antibody_1_040720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, fixed, permeabilized, and intracellularly stained with purified CD152 (CTLA-4) (clone BNI3) (left), or purified mouse IgG2a, κ isotype control (right), followed by biotinylated anti mouse IgG, SAV PE, and CD3 APC. -
PHA-stimulated human peripheral blood mononuclear cells (day-3) were stained with purified anti-human CD152 (CTLA-4) (clone BNI3) (left) or mouse IgG2a, κ isotype control (right), followed by biotinylated anti-mouse IgG and SAV-PE, and then stained with CD3 FITC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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369602 | 100 µg | £113 |
CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.
Product Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
FC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
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Application References
(PubMed link indicates BioLegend citation) -
- Linsley PS, et al. 1992. J. Exp. Med. 176:1595.
- Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.
- Product Citations
- RRID
-
AB_2566610 (BioLegend Cat. No. 369602)
Antigen Details
- Structure
- Ig superfamily and 33 kD.
- Distribution
-
Activated T cells and B cells.
- Function
- Negative regulator of T cell activation.
- Interaction
- Antigen presenting cells, such as dendritic cells.
- Ligand/Receptor
- B7-1 (CD80), B7-2 (CD86).
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143. - Gene ID
- 1493 View all products for this Gene ID
- UniProt
- View information about CD152 on UniProt.org
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