- Clone
- J252D4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BLR1, MDR15
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![J252D4_Pure_CXCR5_Antibody_FC_020713 J252D4_Pure_CXCR5_Antibody_FC_020713](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/J252D4_Pure_CXCR5_Antibody_FC_020713.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified human CD185 (CXCR5, clone J252D4, filled histogram), or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
356901 | 25 µg | £77 | ||||
356902 | 100 µg | £182 |
CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR5-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
- RRID
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AB_2561810 (BioLegend Cat. No. 356901)
AB_2561811 (BioLegend Cat. No. 356902)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions, 42 kD
- Distribution
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Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
- Function
- Mediates cell migration to the B cell follicles in the secondary lymphoid organs
- Ligand/Receptor
- CXCL13
- Cell Type
- B cells, Tfh
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560. - Gene ID
- 643 View all products for this Gene ID
- UniProt
- View information about CD185 on UniProt.org
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
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Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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