Loading...

Purified anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_HIB19_Purified_061807
Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC
  • 1_HIB19_Purified_061807
    Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC
  • 2_HIB19_PURE_CD19_Antibody_IHC-F_091018
    Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD19 antibody (clone HIB19) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # Size Price Quantity Check Availability Save
302201 25 µg £21
Check Availability


Need larger quantities of this item?
Request Bulk Quote
302202 100 µg £42
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
PG - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  2. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  3. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  4. Han SS, et al. 2018. Oncotarget. 9:3292. PubMed
  5. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  6. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  7. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  8. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  9. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  10. Smillie CS et al. 2019. Cell. 178(3):714-730 . PubMed
  11. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  12. Crome SQ, et al. 2017. Nat Med. 1.213888889. PubMed
  13. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  14. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  15. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  16. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  17. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  18. Johnston HE, et al. 2018. Mol Cell Proteomics. 1.247222222. PubMed
  19. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  20. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  21. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  22. Ferdosi SR, et al. 2019. Nat Commun. 1.695833333. PubMed
  23. Afzali B, et al. 2017. Nat Immunol. 18:813. PubMed
  24. Renand A, et al. 2018. J Allergy Clin Immunol. 141:1750. PubMed
  25. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  26. Cerignoli F, et al. 2018. PLoS One. 13:e0193498. PubMed
  27. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  28. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  29. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  30. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  31. Vanshylla K, et al. 2018. Eur J Immunol. 48:441. PubMed
  32. Walker J, et al. 2009. J Immunol. 182:1548. PubMed
  33. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  34. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  35. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  36. Schwabenland M, et al. 2021. Immunity. . PubMed
  37. Martin E, et al. 2020. JCI Insight. :5. PubMed
  38. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  39. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  40. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  41. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  42. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  43. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  44. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  45. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  46. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  47. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  48. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  49. Pugh J, et al. 2021. PeerJ. 9:e12258. PubMed
  50. Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
  51. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  52. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  53. Lartey S, et al. 2020. J Infect Dis. 221:21. PubMed
  54. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  55. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  56. Weinhage T, et al. 2020. J Immunol. 205:56. PubMed
  57. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  58. Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed
  59. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  60. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  61. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  62. Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed
  63. Donnadieu E, et al. 2020. J Immunol. 205:1248. PubMed
  64. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  65. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  66. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  67. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  68. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  69. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
RRID
AB_314231 (BioLegend Cat. No. 302201)
AB_314232 (BioLegend Cat. No. 302202)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 6    Revision Date: 01/06/2023

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account