- Clone
- JES3-9D7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
501401 | 50 µg | £57 | ||||
501402 | 500 µg | £163 |
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS - expressed, recombinant human IL-10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC-P, Neut - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture1-5 or ELISPOT Capture6: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining12.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.
The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10). -
Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
- Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
- Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
- Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
- Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
- Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
- Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
- Stary G, et al. 2011. J. Immunol. 186:103. PubMed
- Mason GM, et al. 2012. PNAS. PubMed
- 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)
- Product Citations
- RRID
-
AB_315167 (BioLegend Cat. No. 501401)
AB_315168 (BioLegend Cat. No. 501402)
Antigen Details
- Structure
- Acid-labile cytokine, dimer, 35-40 kD (Mammalian)
- Bioactivity
- Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
- Cell Sources
- Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
- Cell Targets
- T cells, B cells, mast cells, macrophages
- Receptors
- IL-10R (CDw210)
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385. - Regulation
- Production inhibited by IL-4, IL-10
- Gene ID
- 3586 View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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