- Clone
- C9B7W (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LAG3, LAG-3
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C9B7W_PURE_CD223_Antibody_1_091719 C9B7W_PURE_CD223_Antibody_1_091719](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C9B7W_PURE_CD223_Antibody_1_091719.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD3ε (clone 145-2C11) APC and CD223 (LAG-3) (clone C9B7W) Purified (left) or rat IgG1, κ Purified isotype control (right) followed by anti-Rat IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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125202 | 500 µg | £94 |
CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine CD223-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- Product Citations
- RRID
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AB_961187 (BioLegend Cat. No. 125202)
Antigen Details
- Structure
- Ig superfamily
- Distribution
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T cells and NK cells, as well as T regulatory cells
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
- Gene ID
- 16768 View all products for this Gene ID
- UniProt
- View information about CD223 on UniProt.org
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