- Clone
- M3/38 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M3_38_Pure_072808.jpg M3_38_Pure_072808.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M3_38_Pure_072808.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC -
HeLa cells were stained with purified anti-Galectin 3 (M3/38) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue). -
Immunoprecipitation/Western Blot analysis using anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). 150 µg of HeLa cell lysates were tested at a protein concentration of 1 µg/µL for each sample. Lane 1 was immunoprecipitated with a control antibody and lane 2 was immunoprecipitated with anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). Western blot analysis was performed using anti‐mouse/human Mac‐2 antibody (clone Gal397). -
Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Mac-2 (Galectin-3) antibody (clone M3/38). Proteins were visualized using an HRP Goat anti-rat IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
125401 | 50 µg | £50 | ||||
125402 | 500 µg | £177 |
Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.
Product DetailsProduct Details
- Reactivity
- Mouse,Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Raised against galectin-3 of mouse origin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, WB, IP - Verified
IHC, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.
Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.
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Application References
(PubMed link indicates BioLegend citation) -
- Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
- Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
- Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
- Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
- Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
- Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
- Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
- Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
- Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
- Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
- Product Citations
- RRID
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AB_1134237 (BioLegend Cat. No. 125401)
AB_1134238 (BioLegend Cat. No. 125402)
Antigen Details
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules
- Antigen References
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1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. - Gene ID
- 16854 View all products for this Gene ID 3958 View all products for this Gene ID
- UniProt
- View information about Mac-2 on UniProt.org
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