Loading...

Purified anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1dot4_Pure_070808.jpg
C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_070808.jpg
    C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_Ly6c_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified Ly6C clone HK1.4 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Check Availability Save
128001 50 µg £45
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128002 500 µg £161
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
  2. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  3. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  4. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  5. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  6. Wichaiyo S, et al. 2019. Haematologica. 104:1648. PubMed
  7. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  8. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  9. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  10. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  11. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  12. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  13. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  14. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  15. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  16. Filippini D, et al. 2019. Sci Rep. 9:12286. PubMed
  17. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  18. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  19. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  20. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  21. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  22. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  23. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  24. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  25. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  26. Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed
  27. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  28. De Alessandris S, et al. 2019. J Leukoc Biol. 105:1183. PubMed
  29. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  30. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  31. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  32. Majer O, et al. 2019. Nature. 575:366. PubMed
  33. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  34. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  35. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  36. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  37. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  38. Blriot C, et al. 2021. Immunity. :. PubMed
  39. Zhang C, et al. 2020. Hear Res. 393:107996. PubMed
  40. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  41. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  42. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  43. Hofstee MI, et al. 2021. Pathogens. 10:. PubMed
  44. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  45. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  46. Jones GS, et al. 2020. mSphere. 5:. PubMed
  47. Karhadkar TR, et al. 2021. J Pharmacol Exp Ther. 376:106. PubMed
  48. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  49. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  50. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  51. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  52. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  53. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  54. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  55. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  56. Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed
  57. Hofstee MI, et al. 2021. Eur Cell Mater. 41:774. PubMed
  58. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
  59. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  60. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  61. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  62. Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
  63. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  64. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  65. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  66. Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
  67. Li F, et al. 2020. EMBO J. 39:e103205. PubMed
  68. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  69. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  70. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  71. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  72. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  73. Campisi L, et al. 2022. Nature. 606:945. PubMed
  74. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  75. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
  76. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  77. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
RRID
AB_1134213 (BioLegend Cat. No. 128001)
AB_1134214 (BioLegend Cat. No. 128002)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 10/25/2018

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account