- Clone
- AA10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3), and NF-1 (lane 4) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal tubulin beta 3 (TUBB3) (clone AA10) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. -
Whole cell lysates (15 µg protein) from HEL (negative control), HeLa, NTERA-2, and A549 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-Tubulin Beta 3 (TUBB3) antibody, clone AA10. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TUBB3 mRNA expression levels (source: Human Protein Atlas). -
NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X‐100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti‐Tubulin Beta 3 (TUBB3) (AA10) antibody and followed by DyLight™ 594 goat anti‐mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
657401 | 25 µg | £70 | ||||
657402 | 100 µg | £157 |
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Product DetailsProduct Details
- Reactivity
- Mouse,Rat,Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.25 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
-
AB_2562569 (BioLegend Cat. No. 657401)
AB_2562570 (BioLegend Cat. No. 657402)
Antigen Details
- Structure
- 450 amino acids with predicted molecular weight of 50 kD
- Distribution
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Cytosol
- Function
- Plays important roles in neuronal cell proliferation and differentiation
- Interaction
- Alpha tubulin, kinesin and dynein
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
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1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414. - Gene ID
- 10381 View all products for this Gene ID
- UniProt
- View information about Tubulin beta-3 on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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