- Clone
- S17011E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD16, CD32
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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BALB/c splenocytes were incubated with TruStain FcX™ PLUS (clone S17011E, 0.25µg/106 cells, filled histogram) or TruStain FcX™ (clone 93, 1µg/106 cells, red line histogram) to block the Fc receptors, or were left untreated (black line histogram); then stained with CD90.2 (Thy-1.2, clone 53-2.1) FITC. Note the high background in the untreated cells and the bigger reduction in the background staining when the cells were incubated with TruStain FcX™ Plus compared to TruStain FcX™.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
156603 | 50 µg | £45 | ||||
156604 | 500 µg | £189 |
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Purity
- The antibody was purified by affinity chromatography.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
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For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS for 5-10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µl, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.
- Application Notes
-
Clone S17011E blocks both clone 93 and 2.4G2 also raised against mouse CD16/32
- Product Citations
- RRID
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AB_2783137 (BioLegend Cat. No. 156603)
AB_2783138 (BioLegend Cat. No. 156604)
Antigen Details
- Structure
- Ig superfamily, 40-60 kD
- Distribution
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B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Ligand/Receptor
- IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- UniProt
- View information about CD16/32 on UniProt.org
Related Pages & Pathways
Pages
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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