- Clone
- 26.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Complex Class I-related, HLALS
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![26.5_LEAF_MR1_Antibody_FC_012114 26.5_LEAF_MR1_Antibody_FC_012114](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/26.5_LEAF_MR1_Antibody_FC_012114.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human MR1-transfected Hela cell line was stained with LEAF™ purified MR1 (clone 26.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
361109 | 100 µg | £125 | ||||
361110 | 1 mg | £509 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.
Product DetailsProduct Details
- Reactivity
- Human,Mouse,Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Soluble complexes of MR1 ectodomain and B2M.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
ELISA, IP, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines1-6, detection of folded MR1 conformers3,7, and immunoprecipitation of MR1 from cell lysates7.
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Application References
(PubMed link indicates BioLegend citation) -
- Young MH, et al. 2013. PLoS One. 8:e53789. (Block)
- Leeansyah E, et al. 2013. Blood 121:1124. (Block)
- Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA)
- Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC)
- Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC)
- Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC)
- Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)
- Product Citations
- RRID
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AB_2800999 (BioLegend Cat. No. 361109)
AB_2801000 (BioLegend Cat. No. 361110)
Antigen Details
- Structure
- Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
- Distribution
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MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.
- Function
- Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
- Ligand/Receptor
- α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14.
2. Young MH and Gapin L. 2013. Cell Res. 23:460.
3. Gold MC, et al. 2013. Mucosal Immunol. 6:35.
4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119. - Gene ID
- 3140 View all products for this Gene ID 15064 View all products for this Gene ID 25119 View all products for this Gene ID
- UniProt
- View information about MR1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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