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PE anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PE_021606
C57BL/6 mouse splenocytes stained with 17A2 PE
  • 17A2_PE_021606
    C57BL/6 mouse splenocytes stained with 17A2 PE
Compare all formats See PE spectral data
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100205 50 µg £42
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100206 200 µg £98
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  2. Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.071. PubMed
  3. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  4. Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed
  5. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  8. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  9. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  10. TaSli PN, et al. 2022. J Food Biochem. 46:e14393. PubMed
  11. Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed
  12. Deng P, et al. 2022. J Clin Invest. 132: . PubMed
  13. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  14. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  16. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  17. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  18. Zhao L, et al. 2022. Oncogene. 41:4200. PubMed
  19. Guo D, et al. 2023. Cell Biosci. 13:51. PubMed
  20. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  21. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  22. Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed
  23. Shi Y, et al. 2023. Nat Commun. 14:1884. PubMed
  24. Fiedler T, et al. 2023. J Neuroinflammation. 20:100. PubMed
  25. Li Y, et al. 2023. Front Cell Infect Microbiol. 13:1117230. PubMed
  26. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  27. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  28. Milighetti M, et al. 2023. iScience. 26:106937. PubMed
  29. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  30. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  31. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  32. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  33. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  34. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  35. Ushijima M, et al. 2018. Front Immunol. 9:243. PubMed
  36. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  37. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  38. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  39. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  40. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  41. Bedi B, et al. 2012. Proc Natl Acad Sci U S A. 109:275. PubMed
  42. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  43. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  44. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  45. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  46. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  47. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  48. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  49. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  50. Schmidt D, et al. 2016. PLoS One. 11: 0159059. PubMed
  51. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  52. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  53. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  54. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  55. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  56. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  57. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  58. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  59. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  60. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  61. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  62. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  63. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  64. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  65. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  66. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  67. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  68. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  69. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  70. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  71. Wu J, et al. 2020. Immunity. 53:115. PubMed
  72. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  73. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  74. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  75. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  76. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  77. Goldman E, et al. 2021. Cells. 10:. PubMed
  78. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  79. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  80. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  81. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  82. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  83. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  84. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  85. Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed
  86. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  87. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  88. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  89. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  90. Hu J, et al. 2022. J Neuroinflammation. 19:71. PubMed
  91. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  92. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  93. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  94. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  95. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  96. Pedraza-Zamora C, et al. 2017. Parasite Immunol. 10.1111/pim.12420. PubMed
  97. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  98. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  99. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  100. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  101. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  102. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  103. Huang Z, et al. 2021. Nat Commun. 12:145. PubMed
  104. Cho K, et al. 2021. iScience. 24:103117. PubMed
  105. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  106. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  107. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  108. Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed
  109. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  110. Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed
  111. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  112. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  113. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  114. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  115. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  116. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  117. Zhang T, et al. 2020. Cell. 180(6):1115-1129. PubMed
  118. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  119. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  120. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  121. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  122. Yamamura K, et al. 2017. Nat Commun. 8:13946. PubMed
  123. Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed
  124. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  125. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  126. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  127. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  128. Schneider A, et al. 2014. J Leukoc Biol. 96:857. PubMed
  129. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  130. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  131. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  132. Chen H, et al. 2022. Front Pharmacol. 13:827520. PubMed
  133. Zhou T, et al. 2021. Bioact Mater. 0.734027778. PubMed
  134. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  135. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  136. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  137. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  138. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  139. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  140. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  141. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  142. Fathman J, et al. 2010. Proc Natl Acad Sci U S A. 107:3663. PubMed
  143. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  144. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  145. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  146. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  147. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  148. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  149. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  150. Gawish R, et al. 2022. Elife. 11:. PubMed
  151. Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
  152. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  153. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  154. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  155. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  156. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  157. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
RRID
AB_312662 (BioLegend Cat. No. 100205)
AB_312663 (BioLegend Cat. No. 100206)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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