- Clone
- AA10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AA10_Purified_TUBB3_Antibody_1_WB_92313.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3), and NF-1 (lane 4) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal tubulin beta 3 (TUBB3) (clone AA10) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. -
Whole cell lysates (15 µg protein) from HEL (negative control), HeLa, NTERA-2, and A549 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-Tubulin Beta 3 (TUBB3) antibody, clone AA10. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TUBB3 mRNA expression levels (source: Human Protein Atlas). -
NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X‐100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti‐Tubulin Beta 3 (TUBB3) (AA10) antibody and followed by DyLight™ 594 goat anti‐mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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657401 | 25 µg | 81€ | ||||
657402 | 100 µg | 184€ |
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.25 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
- Application References
- Product Citations
-
- RRID
-
AB_2562569 (BioLegend Cat. No. 657401)
AB_2562570 (BioLegend Cat. No. 657402)
Antigen Details
- Structure
- 450 amino acids with predicted molecular weight of 50 kD
- Distribution
-
Cytosol
- Function
- Plays important roles in neuronal cell proliferation and differentiation
- Interaction
- Alpha tubulin, kinesin and dynein
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414. - Gene ID
- 10381 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Tubulin beta-3
- Specificity Alt (DOES NOT SHOW ON TDS):
- Tubulin β-3 (TUBB3)
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC
- UniProt
- View information about Tubulin beta-3 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Tubulin β-3 (TUBB3) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tubulin Beta 3 (TUBB3) | AA10 | WB,ICC |
Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3) | AA10 | ICFC,IHC-F,3D IHC |
Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) | AA10 | ICFC,IHC-F,3D IHC,SB |
Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) | AA10 | IHC-F,ICC,IHC-P,3D IHC |
Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) | AA10 | WB |
Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3) | AA10 | IHC-F,ICC,SB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tubulin Beta 3 (TUBB3)
Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3)... Whole cell lysates (15 µg protein) from HEL (negative contro... NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for ... -
Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was treated with Bi... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Dissected C57/B6 mouse small intestine was immersed in 4% p... Formalin-fixed, 300 micron-thick mouse kidney section was bl... -
Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was treated with Fi... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Formalin-fixed, 300 micron-thick mouse large intestine secti... -
Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3)
Day-three cultured postnatal C57BL/6 mouse brain cells were ... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Human paraffin-embedded cerebellum tissue slices were prepar... Human paraffin-embedded cerebellum tissue slices were prepar... Formalin-fixed, 300 micron-thick mouse brain (cerebellum) se... -
Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3)
10 µg of total protein extract from HeLa cells was resolved ... -
Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was fixed with 1% p... C57BL/6 mouse frozen brain (cerebellum) tissue was fixed wit...
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