- Clone
- 7D4-6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- KDR, Vascular Endothelial Receptor 2 (VEGF-R2, VEGFR2, VEGFR)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![7D4-6_AF647_VEGFR2_Antibody_FC_042414.jpg 7D4-6_AF647_VEGFR2_Antibody_FC_042414.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/7D4-6_AF647_VEGFR2_Antibody_FC_042414.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HUVEC human endothelial cells were stained with CD309 (clone 7D4-6) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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359909 | 25 tests | 123€ | ||||
359910 | 100 tests | 272€ |
CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human KDR recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Product Citations
-
- RRID
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AB_2563488 (BioLegend Cat. No. 359909)
AB_2563489 (BioLegend Cat. No. 359910)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
- Distribution
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Endothelial cells, embryonic tissues
- Ligand/Receptor
- VEGF-A, VEGF-C, and VEGF-D splice isoforms
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
2. Ferrara N and Gerber HP. 2002. Acta. Haematol. 106:148.
3. Murohara T, et al. 1998. Circulation. 97:99. - Gene ID
- 3791 View all products for this Gene ID
- UniProt
- View information about CD309 on UniProt.org
Related FAQs
Other Formats
View All CD309 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD309 (VEGFR2) | 7D4-6 | FC,ICC |
PE anti-human CD309 (VEGFR2) | 7D4-6 | FC |
PerCP/Cyanine5.5 anti-human CD309 (VEGFR2) | 7D4-6 | FC |
Alexa Fluor® 647 anti-human CD309 (VEGFR2) | 7D4-6 | FC |
PE/Cyanine7 anti-human CD309 (VEGFR2) | 7D4-6 | FC |
Alexa Fluor® 488 anti-human CD309 (VEGFR2) | 7D4-6 | FC |
APC anti-human CD309 (VEGFR2) | 7D4-6 | FC |
PE/Dazzle™ 594 anti-human CD309 (VEGFR2) | 7D4-6 | FC |
TotalSeq™-A0362 anti-human CD309 (VEGFR2) | 7D4-6 | PG |
TotalSeq™-C0362 anti-human CD309 (VEGFR2) | 7D4-6 | PG |
TotalSeq™-B0362 anti-human CD309 (VEGFR2) | 7D4-6 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD309 (VEGFR2)
HUVEC cells were fixed with 1% paraformaldehyde (PFA) and th... HUVEC human endothelial cells were stained with purified CD3... -
PE anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
PerCP/Cyanine5.5 anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
Alexa Fluor® 647 anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
PE/Cyanine7 anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
Alexa Fluor® 488 anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
APC anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
PE/Dazzle™ 594 anti-human CD309 (VEGFR2)
HUVEC human endothelial cells were stained with CD309 (clone... -
TotalSeq™-A0362 anti-human CD309 (VEGFR2)
-
TotalSeq™-C0362 anti-human CD309 (VEGFR2)
-
TotalSeq™-B0362 anti-human CD309 (VEGFR2)
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