Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
Clone
17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Rat IgG2a/Rat IgG2b
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Product Citations
publications
Lineage_Cocktail_AF700_Antibody_FC_1_082112
C57BL/6 mouse bone marrow cells were stained with CD117 FITC and Alexa Fluor® 700 lineage cocktail (top plot) or Alexa Fluor® 700 isotype controls (bottom plot).
  • Lineage_Cocktail_AF700_Antibody_FC_1_082112
    C57BL/6 mouse bone marrow cells were stained with CD117 FITC and Alexa Fluor® 700 lineage cocktail (top plot) or Alexa Fluor® 700 isotype controls (bottom plot).
  • Lineage_Cocktail_AF700_Antibody_FC_2_082112
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133313 25 tests 235€
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Alexa Fluor® 700 Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Alexa Fluor® 700 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

 

Materials provided:
1. Alexa Fluor® 700 anti-mCD3/ Alexa Fluor® 700 anti-mLy-6G(Ly-6C)/ Alexa Fluor® 700 anti-mCD11b/ Alexa Fluor® 700 anti-mCD45R(B220)/ Alexa Fluor® 700 anti-mTer-119
2. Alexa Fluor® 700 Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
This two-reagent set contains Alexa Fluor® 700 conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  2. Zhou Y, et al. 2022. Elife. 11:. PubMed
  3. De S, et al. 2018. Development. 145. PubMed
  4. Takagaki S, et al. 2019. Sci Rep. 9:7133. PubMed
  5. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  6. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
  7. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  8. Felker S, et al. 2022. JCI Insight. 7:. PubMed
  9. Peltzer N, et al. 2018. Nature. 557:112. PubMed
RRID
AB_2715571 (BioLegend Cat. No. 133313)

Antigen Details

Distribution

T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Cell Type
B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Other Formats

View All Lineage Reagents Request Custom Conjugation
Description Clone Applications
Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Pacific Blue™ anti-mouse Lineage Cocktail 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Brilliant Violet 421™ anti-mouse Lineage Cocktail 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 4    Revision Date: 07/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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