- Clone
- W18029B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GSDMD, DF5L, DFNA5L, GSDMC1,FKSG10, Gadermin domain-containing protein 1
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18029A_PURE_GasderminD_Antibody_092220 W18029A_PURE_GasderminD_Antibody_092220](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18029B_PURE_Gasdermin-D_Antibody_1_101520.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Gasdermin-D antibody (clone W18029B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Whole cell extracts (250 µg total protein) prepared from PC-3 cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, λ isotype ctrl antibody (Cat. No. 403801) or purified anti-Gasdermin-D antibody (clone W18029B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rat control antibody against a separate epitope of Gasdermin-D. Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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939701 | 25 µg | 95€ | ||||
939702 | 100 µg | 235€ |
Gasdermin D (N-terminus) is a member of the Gasdermin family of pore-forming proteins involved in pyroptosis. Gasdermin-D is vital to inducing pyroptotic cell death in response to pathogen and damage-associated molecular patterns, thus performing a role crucial to innate immune defense. Gasdermin-D is localized to the cytosol in an inactive state but, following cleavage into N and C-terminal fragments by inflammatory caspases Caspase-1, 4, or 5, the N-terminal fragment translocates to the plasma membrane where it forms large oligomeric pores. Pore-formation results in a disruption in the cell’s osmotic potential and subsequent cell lysis. Secretion of pro-inflammatory cytokines IL-1β and IL-18 from bone marrow dendritic cells (BMDCs), bone marrow derived macrophages (BMDMs), and neutrophils is also dependent upon Gasdermin-D, both through release by cell lysis and by direct passage through pores formed by its N-terminal fragment in the plasma membrane. Gasdermin-D exhibits bactericidal properties through the binding of its N-terminus to the cardiolipin of bacterial cell membranes and subsequent membrane perforation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human Gasdermin-D corresponding to the N-terminus of the protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting and immunoprecipitation the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone was tested for ICC using NTERA-2 (negative control) and PC-3 (positive control) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in weak staining with high background.
W18029B is predicted to recognize the pro-pyroptotic N-terminal fragment of cleaved Gasdermin-D. The immunogen used to generate this clone is fully contained within the sequence of the N-terminal fragment. - RRID
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AB_2888893 (BioLegend Cat. No. 939701)
AB_2888893 (BioLegend Cat. No. 939702)
Antigen Details
- Structure
- Gasdermin-D is a 484 amino acid protein with an expected molecular weight of 53 kD.
- Distribution
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Ubiquitously expressed/Cytosol and plasma membrane
- Function
- Pyroptosis, inflammatory response
- Biology Area
- Innate Immunity, Mitochondrial Function
- Molecular Family
- Innate Immune Signaling
- Antigen References
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1. Heilig R, et al. 2017. Eur. J. Immunol. 48:584.
2. Kambara H, et al. 2018. Cell Reports. 22:2924.
3. Mulvihill E, et al. 2018. EMBO J. 37:98321
4. Shi J, et al. 2015. Nature. 526:660. - Gene ID
- 79792 View all products for this Gene ID
- UniProt
- View information about Gasdermin-D on UniProt.org
Related FAQs
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Gasdermin-D (N-terminus) Antibody | W18029B | WB,IP |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Gasdermin-D (N-terminus) Antibody
Whole cell extracts (15 µg total protein) from the indicated... Whole cell extracts (250 µg total protein) prepared from PC-...
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