Purified anti-SAPAP (pan reactive) Antibody (Previously Covance catalog# MMS-5176)

Pricing & Availability
Clone
N127/31 (See other available formats)
Regulatory Status
RUO
Other Names
Disks large-associated protein 1, DAP-1, rGKAP, SAPAP1, PSD-95/SAP90-binding protein 1, SAP90/PSD-95-associated protein 1, guanylate kinase associated protein, guanylate kinase-associated protein, Gkap
Previously
Covance Catalog# MMS-5176
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N127slash31_Purified_SAPAP_Antibody_WB_042418
Western blot of purified anti-SAPAP (pan reactive) antibody (clone N127/31). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N127slash31_Purified_SAPAP_Antibody_WB_042418
    Western blot of purified anti-SAPAP (pan reactive) antibody (clone N127/31). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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832601 100 µL 132€
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Description

SAPAP is also known as DAP-1 or GKAP,  encoded by the DLGAP1 gene.  It is enriched in the postsynaptic membrane and is responsible for connecting PSD-95 and SHANK, which serves as an adapter protein that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 772-992 (C-terminus) of rat SAPAP1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).
 

Product Citations
  1. Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed
RRID
AB_2564958 (BioLegend Cat. No. 832601)

Antigen Details

Structure
Expected MW: 120 kD (SAPAP2), 110/105/108 kD (SAPAP1/3/4)
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
9229 View all products for this Gene ID
UniProt
View information about SAPAP pan reactive on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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