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Ultra-LEAF™ Purified anti-human CD337 (NKp30) Antibody

Pricing & Availability
Clone
W20238A (See other available formats)
Regulatory Status
RUO
Other Names
NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20238A_ULEAF_CD337_Block_041624
Biotinylated recombinant human NKp30 (black circles) binds to immobilized recombinant human B7-H6-Fc chimera (Cat. No. 794004). Ultra-LEAF™ purified anti-human CD337 (NKp30) (clone W20238A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have effect. This antibody blocks the binding of 1.2 µg/mL biotinylated human NKp30 to 1 µg/mL immobilized recombinant human B7-H6-Fc chimera. ND50 range: 0.6-3 µg/mL.
  • W20238A_ULEAF_CD337_Block_041624
    Biotinylated recombinant human NKp30 (black circles) binds to immobilized recombinant human B7-H6-Fc chimera (Cat. No. 794004). Ultra-LEAF™ purified anti-human CD337 (NKp30) (clone W20238A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have effect. This antibody blocks the binding of 1.2 µg/mL biotinylated human NKp30 to 1 µg/mL immobilized recombinant human B7-H6-Fc chimera. ND50 range: 0.6-3 µg/mL.
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380605 100 µg 364€
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Description

CD337, also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3, is recognized by the W20238A monoclonal antibody. CD337, a member of the natural cytotoxicity receptor family is a type I transmembrane protein with one immunoglobulin-like domain and a molecular weight of 30 kD. It exists in six isoforms from alternative splicing. NKp30 is expressed on resting and activated NK cells and enhances NK cell cytolysis of tumor cells that are deficient in MHC class I molecules. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NKp30
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1.2 µg/mL recombinant biotinylated human NKp30 to immobilized 1 µg/mL recombinant human B7-H6-Fc chimera (Cat. No. 794004).  ND50 range: 0.6 - 3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain
Distribution

Resting and activated NK cells

Function
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction
CD59, TCRζ
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1.  Pende D, et al. 1999. J Exp Med. 190:1505-16.
Gene ID
259197 View all products for this Gene ID
UniProt
View information about CD337 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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