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APC anti-human CD80 Antibody

Pricing & Availability
Clone
2D10 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD80.1
Other Names
B7-1, B7, BB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2D10_APC_040610
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 APC
  • 2D10_APC_040610
    Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 APC
See APC spectral data
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305219 25 tests 84€
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305220 100 tests 199€
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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Mertens C, et al. 2018. Oncoimmunology. 7:e1408751. PubMed
  2. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  3. Katerina Gkirtzimanaki et al. 2018. Cell reports. 25(4):921-933 . PubMed
  4. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  5. Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed
  6. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  7. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  8. Redka DS, et al. 2018. Mol Biol Cell. 29:53. PubMed
  9. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  10. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  11. Škrnjug I, et al. 2014. PLoS One. 9:110150. PubMed
  12. Brummelman J, et al. 2015. Infect Immun . 83:227. PubMed
  13. Hashimoto M, et al. 2015. J Immunol. 95: 4341 - 4350. PubMed
  14. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  15. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  16. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  17. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  18. Jost M, et al. 2021. eLife. 10:00. PubMed
  19. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  20. Chaumonnot K, et al. 2021. Cell Death Dis. 12:114. PubMed
  21. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  22. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  23. Murugesan G, et al. 2022. Biomedicines. 10:. PubMed
  24. Amo L, et al. 2022. Front Immunol. 13:835527. PubMed
  25. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  26. Zhang Z, et al. 2022. Clin Transl Med. 12:e1072. PubMed
  27. Xu C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:327. PubMed
  28. Siepe DH, et al. 2022. Elife. 11:. PubMed
RRID
AB_2291403 (BioLegend Cat. No. 305219)
AB_2076147 (BioLegend Cat. No. 305220)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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