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- Clone
- TC11-8H4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 507001 | 50 µg | 95€ | ||||
| 507002 | 500 µg | 256€ | ||||
IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-8H4 antibody antibody reacts with mouse interleukin-17 (IL-17).
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IL-17A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection, ELISPOT Detection
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 1000 to 15.63 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA2 and ELISPOT3 Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10 antibody (Cat. No. 506901) as the capture antibody, and with recombinant mouse IL-17 (Cat. No. 564101) as the standard.
- Application References
-
- Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
- Herrada A, et al. 2010. J. Immunol. 184:191
- Nekrasova T, et al. 2005. J. Immunol. 175:2734.
- Product Citations
- RRID
-
AB_315465 (BioLegend Cat. No. 507001)
AB_315466 (BioLegend Cat. No. 507002)
Antigen Details
- Structure
- Cytokine; dimer; 15 kD (Mammalian)
- Bioactivity
- Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
- Cell Sources
- CD4+ memory T cells
- Cell Targets
- Fibroblasts, epithelial and endothelial cells, stromal cells
- Receptors
- IL-17R (CD217)
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006 Nat. Rev. Immunol. 6(4):329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237(2):123. - Gene ID
- 16171 View all products for this Gene ID
- UniProt
- View information about IL-17A on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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