Brilliant Violet 605™ Streptavidin

Product Details

Reactivity

Human,Mouse,Rat,All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

Streptavidin was conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

0.1 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 605™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 605™ conjugation. For immunofluorescent staining, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 605™ is 340 kD and Streptavidin alone is 52 kD.

Application References

1. Altin JA, et al. 2014. PNAS. 111:2067. PubMed
2. Perez-Quintero LA, et al. 2014. J Exp Med. 211:727. PubMed
3. Cartwright EK, et al. 2014. J. Immunol. 192:4666. PubMed
4. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
5. Arlehamnn CL, et al. 2014. J Immunol. 193:2931. PubMed
6. Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed

Product Citations

1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
2. Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed
3. Chen G, et al. 2019. Curr Protoc Cell Biol. 82:e65. PubMed
4. Pineault KM, et al. 2019. Nat Commun. 10:3168. PubMed
5. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
6. Delacher M, et al. 2020. Immunity. 52:295. PubMed
7. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
8. Ko KH, et al. 2018. Sci Rep. 8:16905. PubMed
9. Altin J, et al. 2014. Proc Natl Acad Sci U S A. 111:2067. PubMed
10. Pérez-Quintero L, et al. 2014. J Exp Med. 211:727. PubMed
11. Cartwright E, et al. 2014. J Immunol. 192:4666. PubMed
12. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
13. Arlehamn C, et al. 2014. J Immunol. 193:2931. PubMed
14. Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed
15. Kolan S, et al. 2015. PLoS One. 10: 0134113. PubMed
16. Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed
17. Lainé A, et al. 2015. J Immunol. 195: 1791-1803. PubMed
18. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
19. Schmid M, et al. 2016. PLoS One. 12: 1005676. PubMed
20. Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
21. Ma C, et al. 2016. J Exp Med. 213: 1589 - 1608. PubMed
22. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
23. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
24. Pascual G, et al. 2016. Nature. 541:41-45. PubMed
25. Mehedi M, et al. 2016. PLoS Pathog. 12:e1006062. PubMed
26. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
27. Valletta S, et al. 2020. Nat Commun. 3.288194444. PubMed
28. Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed
29. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
30. Hutter K, et al. 2020. FEBS J. . PubMed
31. Geng G, et al. 2021. Elife. 10: . PubMed
32. Wells DK, et al. 2020. Cell. 183:818. PubMed
33. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
34. Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
35. , et al. 2021. Eur J Immunol. 51:2708. PubMed
36. Pascual G, et al. 2021. Nature. 599:485. PubMed
37. Cao Y, et al. 2022. Nature. :. PubMed
38. Han YH, et al. 2021. Science. 373:. PubMed
39. Liu R, et al. 2021. Mol Cell Biol. 41:e0025121. PubMed
40. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
41. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
42. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
43. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
44. Cao Y, et al. 2023. Nature. 614:521. PubMed
45. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
46. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
47. Nagashima K, et al. 2022. J Immunol. 209:5. PubMed
48. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
49. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
50. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
51. Barisas DAG, et al. 2023. PLoS Biol. 21:e3001746. PubMed
52. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed

Antigen Details

Gene ID

NA

UniProt

View information about Biotin on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis