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Brilliant Violet 785™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_BV785_061812
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 785™.
  • 17A2_BV785_061812
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 785™.
See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability Save
100231 125 µL 176€
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100232 50 µg 219€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  2. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  3. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  4. Wang W, et al. 2019. Cell Rep. 28:486. PubMed
  5. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  6. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  7. Lofano G, et al. 2015. J Immunol. 195: 1617-1627. PubMed
  8. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  9. Rouleau N, et al. 2020. Immunohorizons. 0.695833333. PubMed
  10. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  11. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  12. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  13. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  14. Glassman CR, et al. 2021. eLife. 10:00. PubMed
  15. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  16. Shreeve N, et al. 2021. Immunity. 54(6):1231-1244.e4. PubMed
  17. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  18. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  19. Carter TJ, et al. 2021. Small. 17:e2005241. PubMed
  20. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed
  22. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  23. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  24. Li H, et al. 2020. Nature. 584:274. PubMed
  25. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  27. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  28. Nanishi E, et al. 2022. Commun Biol. 5:790. PubMed
  29. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  31. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  32. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  33. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  34. Arruda DC, et al. 2022. J Control Release. 350:228. PubMed
  35. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  36. Caradonna TM, et al. 2022. Cell Rep. 41:111628. PubMed
  37. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  38. Liu J, et al. 2023. Nat Commun. 14:2806. PubMed
  39. Borbet TC, et al. 2023. iScience. 26:106810. PubMed
RRID
AB_11218805 (BioLegend Cat. No. 100231)
AB_2562554 (BioLegend Cat. No. 100232)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 10/21/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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