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FITC anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
Clone
145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
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Product Citations
publications
A_FITC_Lineage_042909.jpg
C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • A_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • B_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC isotype control cocktail and CD34 (clone RAM34) Alexa Fluor® 647
Cat # Size Price Quantity Check Availability Save
133301 25 tests 306€
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133302 100 tests 516€
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. FITC anti-mCD3ε/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 tests
2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 tests


Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Formulation
This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation
The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References
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  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
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Product Citations
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  14. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  15. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
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  25. Broxmeyer HE, et al. 2021. Blood Cells Mol Dis. 86:102492. PubMed
  26. Broxmeyer HE, et al. 2022. Stem Cell Rev Rep. 18:2513. PubMed
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  28. Sakae H, et al. 2023. Cells. 12:. PubMed
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  31. Tikka C, et al. 2023. Front Immunol. 14:1026368. PubMed
  32. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
RRID
AB_10697030 (BioLegend Cat. No. 133301)
AB_10697030 (BioLegend Cat. No. 133302)

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Go To Top Version: 2    Revision Date: 07/09/2021

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