Loading...

Pacific Blue™ anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5E2_PacBlue_CD14_Antibody_110316
Human peripheral blood monocytes stained with clone M5E2 Pacific Blue™ or with an isotype control (open histogram).
  • M5E2_PacBlue_CD14_Antibody_110316
    Human peripheral blood monocytes stained with clone M5E2 Pacific Blue™ or with an isotype control (open histogram).
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
301816 25 µg 90€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
301828 100 tests 212€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
301815 100 µg 215€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
  2. Lee J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183594. PubMed
  3. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  4. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  5. Crane A, et al. 2018. Alzheimers Dement. 14:243. PubMed
  6. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  7. Rius C, et al. 2018. J Immunol. 200:2263. PubMed
  8. Longino NV, et al. 2019. Cancer Immunol Res. 7:1727. PubMed
  9. Ilinykh PA, et al. 2020. Cell Host & Microbe. 27(6):976-991. PubMed
  10. Santer D, et al. 2010. J Immunol. 185:4738. PubMed
  11. Alkanani A, et al. 2012. Diabetes. 61:2525. PubMed
  12. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
  13. Li H, et al. 2015. J Infect Dis. 211:1717. PubMed
  14. Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
  15. Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed
  16. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  17. Bernardes JP, et al. 2020. Immunity. 53:1296. PubMed
  18. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  19. van Kempen TS, et al. 2020. Eur J Immunol. 50:1209. PubMed
  20. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  21. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  22. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  23. Dolton G, et al. 2022. Cell. 185:2936. PubMed
  24. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
RRID
AB_493164 (BioLegend Cat. No. 301816)
AB_2275670 (BioLegend Cat. No. 301828)
AB_493163 (BioLegend Cat. No. 301815)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 5    Revision Date: 11/03/2016

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account