- Clone
- GIR-94 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFN-γR, IFN-γRα, IFN-gamma R1, CDw119
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![GIR-94 GIR-94](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/GIR94.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with biotinylated GIR-94 and detected with Sav-PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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308703 | 25 tests | 88€ | ||||
308704 | 100 tests | 196€ |
CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IFN-γRα, purified from human placenta
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: Western blotting3,4, immunoprecipitation3,4, immunofluorescence microscopy6. GIR-94 antibody does not inhibit IFN-? binding to IFN-? R a chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308704) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308706) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
- Application References
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- Sheehan K, et al. 1988. J. Immunol. 140:4231.
- Jouanguy E, et al. 2000. J. Clin. Invest. 105:1429.
- Kaplan D, et al. 1996. J. Biol. Chem. 271:9. (WB IP)
- Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (WB IP)
- Choi JC, et al. 2007. J. Immunol. 178:1598.
- Humlicek AL, et al. 2007. J. Immunol. 178:6395. (IF)
- Aomatsu M, et al. 2013. Biochem Biophys Res Commun. 6:1714. PubMed
- Product Citations
- RRID
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AB_2121591 (BioLegend Cat. No. 308703)
AB_2121590 (BioLegend Cat. No. 308704)
Antigen Details
- Structure
- Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
- Distribution
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Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
- Function
- Activates Jak1, Jak2, STAT1, host defense
- Ligand/Receptor
- IFN-γ
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Calderon J, et al. 1988. P. Natl. Acad. Sci. USA 85:4837.
2. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
3. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835. - Gene ID
- 3459 View all products for this Gene ID
- UniProt
- View information about CD119 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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