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PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_PECyanine7_CD117_Antibody_1_061324
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b (clone M1/70) FITC and anti-mouse CD117 (c-Kit) (clone 2B8) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
  • 2B8_PECyanine7_CD117_Antibody_1_061324
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b (clone M1/70) FITC and anti-mouse CD117 (c-Kit) (clone 2B8) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
See PE/Cyanine7 spectral data
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105813 25 µg 70€
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105814 100 µg 169€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  21. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  22. Shibata F, et al. 2009. Stem Cells. 27:183. PubMed
  23. Shiozawa Y, et al. 2010. PLoS One. 5:e10853. PubMed
  24. Joseph J, et al. 2012. Mol Cancer Res. 0.681944444. PubMed
  25. Xu H, et al. 2012. Blood. 120:3089. PubMed
  26. Nakano N, et al. 2008. J Allergy Clin Immunol. 123:74. PubMed
  27. Obata-Ninomiya K, et al. 2013. J Exp Med. 210:2583. PubMed
  28. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
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  34. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  35. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  36. Sulsenti R, et al. 2021. Frontiers in Immunology. 12:622001. PubMed
  37. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  38. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
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  49. Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed
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  51. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
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  53. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  54. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  55. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  56. Gao X, et al. 2021. Nature. 589:591. PubMed
  57. Zhao D, et al. 2021. Innate Immun. 27:533. PubMed
  58. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  59. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  60. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  61. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
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  65. Mesa-Nuñez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed
  66. Tung LT, et al. 2021. Cell Death Dis. 12:923. PubMed
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RRID
AB_313222 (BioLegend Cat. No. 105813)
AB_313223 (BioLegend Cat. No. 105814)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org
Go To Top Version: 2    Revision Date: 06/28/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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