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Purified anti-human CD206 (MMR) Antibody

Pricing & Availability
Clone
15-2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype
Mouse IgG1, κ
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Product Citations
publications
15-2_Purified_090707
GM-CSF-stimulated (day-3) human monocytes stained with purified 15-2, followed by anti-mouse IgG FITC
  • 15-2_Purified_090707
    GM-CSF-stimulated (day-3) human monocytes stained with purified 15-2, followed by anti-mouse IgG FITC
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321101 25 µg 52€
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321102 100 µg 95€
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Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human mannose receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Application References
  1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
  2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
  3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Trumpi K, et al. 2018. Oncotarget. 3. PubMed
  3. Behrens NE, et al. 2019. MBio. 10:2. PubMed
  4. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  5. Laudanski K, et al. 2018. J Transl Med. 0.765972222. PubMed
  6. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  7. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  8. Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
  9. Brasil da Costa FH, et al. 2020. PLoS One. 15:e0230354. PubMed
  10. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
  11. Gardet A, et al. 2010. J Immunol. 185:5577. PubMed
  12. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  13. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  14. Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
  15. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  16. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  17. Lee SE, et al. 2021. JCI Insight. 6:. PubMed
  18. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  19. Azari S, et al. 2021. MBio. 12:e0184921. PubMed
  20. Muir LA, et al. 2022. JCI Insight. 7:. PubMed
  21. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  22. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  23. Gallucci L, et al. 2023. EMBO Rep. 24:e56818. PubMed
RRID
AB_571922 (BioLegend Cat. No. 321101)
AB_571923 (BioLegend Cat. No. 321102)

Antigen Details

Structure
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution

Macrophages, dendritic cells, hepatic and lymphatic endothelial cells

Function
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor
Mannose, fucose, N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.

Gene ID
4360 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org
Go To Top Version: 2    Revision Date: 01/03/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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