Loading...

Purified anti-human CD335 (NKp46) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
9E2_Pure_021009.jpg
Human peripheral blood lymphocytes stained with purified 9E2, followed by anti-mouse IgG FITC
  • 9E2_Pure_021009.jpg
    Human peripheral blood lymphocytes stained with purified 9E2, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Check Availability Save
331902 100 µg 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations
  1. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  2. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  3. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  4. Sanz–Ortega L, et al. 2019. Front Immunol. 1.85625. PubMed
  5. Capuano C, et al. 2020. Cancer Immunol Immunother. 69:501. PubMed
  6. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  7. Jarahian M, et al. 2009. J Virol. 83:8108. PubMed
  8. Rother S, et al. 2015. PLoS One. 10: 0135682. PubMed
  9. Nguyen R, et al. 2021. Cancer Immunol Immunother. 70:721. PubMed
  10. Gao H, et al. 2021. J Virol. :. PubMed
  11. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  12. Li J, et al. 2021. Haematologica. 106:3115. PubMed
  13. Damele L, et al. 2021. Cancers (Basel). 13:. PubMed
  14. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  15. Dorsch M, et al. 2020. Eur J Immunol. 50:656. PubMed
  16. Aarsund M, et al. 2022. Cancer Immunol Immunother. 71:2227. PubMed
RRID
AB_1027637 (BioLegend Cat. No. 331902)

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org
Go To Top Version: 2    Revision Date: 04/15/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account