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Purified anti-mouse CD106 Antibody

Pricing & Availability
Clone
429 (MVCAM.A) (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
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Product Citations
publications
429_Pure_072808.jpg
C57BL/6 mouse bone marrow myeloid cells stained with 429 purified, followed by anti-rat IgG FITC.
  • 429_Pure_072808.jpg
    C57BL/6 mouse bone marrow myeloid cells stained with 429 purified, followed by anti-rat IgG FITC.
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105701 50 µg 48€
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105702 500 µg 187€
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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tedford K, et al. 2017. Nat Commun. . 10.1038/s41467-017-02482-4. PubMed
  2. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  3. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  4. Wang C, et al. 2014. J Exp Med. 211:395. PubMed
  5. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  6. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  7. Kimura T, et al. 2021. Development. 148: . PubMed
  8. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  9. Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed
RRID
AB_313202 (BioLegend Cat. No. 105701)
AB_313203 (BioLegend Cat. No. 105702)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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