In Parkinson's disease (PD) patients, certain MHC alleles have been found to be more prevalent than others. Sulzer et al. wanted to determine whether PD is associated with an antigen-specific T cell response to α-Synuclein, a hallmark PD protein that aggregates and forms Lewy Bodies. Sulzer produced an α-Synuclein peptide library and tested PBMCs from PD patients. They measured IFN-γ production from Th1 and CD8+ T cells, as well as IL-5 secretion from Th2 cells.

The Y39 and S129 epitope regions elicited the most immune responses from the listed MHC I and II alleles below. These epitopes are sensitive to PD-promoting mutations, and the alleles are present at higher frequencies in PD patients compared to healthy controls. This indicates that α-Synuclein in the cerebrospinal fluid could be captured by antigen presenting cells, displayed by PD-associated MHC alleles, and promote adaptive immune responses.
 

Adapted from Sulzer, et al. 2017. Nature. 546:656. Pubmed

BioLegend offers several antibodies to detect phosphorylated and aggregated/fibrillar forms of α-Synuclein. In addition, we provide Flex-T™, a unique tetramer system that allows peptides of interest to be exchanged in the MHC groove.
Clone Target Recognition
4B12/Synuclein Monomers, Fibrils
A15119B Phospho (Tyr39)
A15126D 117-122 aa
A15127A Truncated C-terminus, x-122 aa
P-syn/81A Phospho (Ser129)
Syn-F1 Fibrils
Syn-O2 Oligomers
Syn-O3 Oligomers
Syn-O4 Oligomers
Description
LEGEND MAX™ Human α-Synuclein ELISA Kit with Pre-coated Plate
Description
Custom HLA Monomers
Custom HLA Tetramers
HLA Class I ELISA Control
HLA-A*01:01 Monomer UVX
HLA-A*02:01 Monomer UVX
HLA-A*03:01 Monomer UVX
HLA-A*11:01 Monomer UVX
HLA-B*07:02 Monomer UVX
HLA-B*08:01 Monomer UVX
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