APC/Fire™ 750 Streptavidin

Pricing & Availability
Other Names
Streptavidin-Allophycocyanin/Fire™, SAv-APC/FireFire™ 750, streptavidin APC/FireFire™ 750
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Strepavidin_APCFIRE_Antibody_FC_110816
Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
  • Strepavidin_APCFIRE_Antibody_FC_110816
    Human peripheral blood lymphocytes were stained with biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-APC/Fire™ 750.
See APC/Fire™ 750 spectral data
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Fire™ 750 is useful for detecting biotinylated antibodies. The excitation of APC/Fire™ 750 by 600-635 nm laser light induces a fluorescence emission maximum of 787 nm.

Product Details
Cat # Size Price Quantity Avail. Save
405250 100 µg $175.00
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Technical data sheet

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Fire™ 750 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin APC/Fire™ 750 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Fire™ 750 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

*APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Streptavidin-Allophycocyanin/Fire™ 750 (APC/Fire™ 750 ) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Fire™ 750 is 350 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Maresz K, et al. 2005. J. Neurochemistry 95:437.
2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed.
3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
5. Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed
6. Cao YA, et al. 2008. Blood PubMed
7. Garidou L, et al. 2009. J. Virol. PubMed
8. Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed
9. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed.
10. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
11. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
12. Prince J, et al. 2013. J. Immunol. 191:4709. PubMed
13. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
14. Orre M, et al. 2014. Neurbiol Aging. 35:1. PubMed
15. Barbera M, et al. 2014. Gut. 64:11. PubMed
16. Zhang Y, et al. 2014. Infect Immun. 82:1698. PubMed
17. Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed
18. Barbera M, et al. 2015. Gut. 64:11. PubMed
19. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed

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