Helix NP™ Green

Pricing & Availability
Other Names
Ave. Rating
0 reviews
Helix_NP_Green_1_FC_112916
One-day-old C57BL/6 mouse splenocytes were stained with 5 µM Helix NP™ Green and APC Annexin V with no wash.
  • Helix_NP_Green_1_FC_112916
    One-day-old C57BL/6 mouse splenocytes were stained with 5 µM Helix NP™ Green and APC Annexin V with no wash.
  • Helix_NP_Green_2_IF_112916
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL of Alexa Fluor® 647 anti-Cytokeratin (pan reactive) antibody (red) in blocking buffer overnight followed by 1:500 dilution of Helix NP™ Green (green) and Flash Phalloidin™ Red 594 (blue) staining for 15 minutes at room temperature. The image was captured with a 60X objective.
  • Helix_NP_Green_3_IHCF_112916
    Mouse frozen cerebellum tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for one hour. Then the tissue was intracellularly stained with 1:500 dilution of Helix NP™ Green (green) for 15 minutes at room temperature. The image was captured with a 20X obj
See high resolution IF/IHC-F data...
Description

Helix NP™ Green is a green fluorescent, nucleic acid stain. It is impermeant to live cells and thus can be used for discrimination of live and dead cells. It is optimally excited at 495 nm with an emission at 519 nm, which can be detected in the Alexa Fluor® 488 or FITC channel.

Product Details
Cat # Size Price Quantity Avail. Save
425303 250 µl $180.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Technical data sheet

Product Details

Preparation
Helix NP™ Green is supplied at 250 µl per vial.
Concentration
5.0 mM
Storage & Handling
Upon receipt, store at -20°C.
Application

FC - Quality tested
IF, IHC-F - Validated

Recommended Usage

For flow cytometric staining, the recommended use of this reagent is 1-5 µM. For immunofluorescence microscopy and immunohistochemisty on frozen tissue sections, the recommended dilution is 1:500-1:10,000.

Application Notes

Helix NP™ Green is a cell-impermeant nucleic acid probe suitable for use as a viability dye. It is also useful for assessing DNA content and cell cycle following cell fixation and permeabilization. It is a green fluorescent dye with an excitation/emission max of 495 nm/519 nm that can be detected in the FITC/ Alexa Fluor® 488 channels. For use in viability assays, the optimal concentration can range from 1 µM - 5 µM. For use in cell cycle, the optimal concentration can range from 2.5 µM - 10 µM.

Protocol for viability staining using Helix NP™ Green:

1. Isolate cells following protocol of choice.
2. Dilute Helix NP™ Green to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. We have observed good results in flow cytometry at 1-5 µM.
3. Following the addition of Helix NP™ Green to cells, do not wash, cells are ready for acquisition.
4. Analyze cells on a cytometer equipped with the 488nm blue laser.

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/02/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Request an Account