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Pacific Blue™ anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
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Product Citations
publications
G10F5_PB_CD66b_Antibody_FC_032014
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
  • G10F5_PB_CD66b_Antibody_FC_032014
    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
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305111 25 tests $136
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305112 100 tests $335
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gilchuk P, et al. 2020. Immunity. 52:388. PubMed
  2. Barrett JR, et al. 2021. Nat Med. 27:279. PubMed
  3. Kaplonek P, et al. 2022. Sci Transl Med. 14:eabm2311. PubMed
  4. Davies LRL, et al. 2022. Sci Transl Med. 14:eabm4065. PubMed
  5. Bernshtein B, et al. 2022. Cell Rep. 40:111216. PubMed
  6. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  7. Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed
  8. Spatola M, et al. 2022. J Infect Dis. 226:738. PubMed
  9. Bartsch YC, et al. 2022. Sci Transl Med. 14:eabn9237. PubMed
  10. Herman JD, et al. 2022. Cell Rep Med. 3:100811. PubMed
  11. Loos C, et al. 2023. NPJ Vaccines. 8:34. PubMed
  12. Boudreau CM, et al. 2023. Cell Rep Med. 4:100975. PubMed
  13. Roy V, et al. 2023. NPJ Vaccines. 8:39. PubMed
  14. Adams LE, et al. 2023. Cell Rep. 42:112326. PubMed
  15. Bartsch YC, et al. 2023. iScience. 26:106582. PubMed
  16. Kaplonek P, et al. 2023. Nat Immunol. . PubMed
  17. Boudreau CM, et al. 2020. J Clin Invest. 130:662. PubMed
  18. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Gorman MJ, et al. 2021. Cell Rep Med. 2:100405. PubMed
  21. Jundi B, et al. 2021. JCI Insight. 6:e148866. PubMed
  22. House RV, et al. 2022. PLoS One. 17:e0267796. PubMed
  23. Herman JD, et al. 2021. Nat Commun. 12:6853. PubMed
  24. Williamson LE, et al. 2021. Cell. 184:4430. PubMed
  25. Gunn BM, et al. 2021. Immunity. 54(4):815-828.e5. PubMed
  26. Gunn BM et al. 2018. Cell host & microbe. 24(2):221-233 . PubMed
  27. Chen RE, et al. 2021. Med (N Y). 2:1327. PubMed
  28. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  29. Offersen R, et al. 2020. Cell Rep. 33:108502. PubMed
  30. Cadena AM, et al. 2021. Nat Commun. 12:1474. PubMed
  31. Minassian AM, et al. 2021. Med (N Y). 2:701. PubMed
  32. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  33. Lu LL et al. 2016. Cell. 167(2):433-443 . PubMed
  34. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  35. Dolatshahi S, et al. 2022. Sci Rep. 12:14937. PubMed
  36. Krampert L, et al. 2021. Front Immunol. 12:712948. PubMed
  37. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  38. Zelter T, et al. 2022. EMBO Rep. 23:e53641. PubMed
  39. Pullen KM, et al. 2021. Cell Rep. 37:109959. PubMed
  40. Bartsch YC, et al. 2021. Nat Commun. 12:1018. PubMed
  41. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  42. Jennewein MF, et al. 2020. Cell. 178(1):202-215.e14.. PubMed
  43. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  44. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  45. Om K, et al. 2020. PLoS Pathog. 16:e1008764. PubMed
  46. Ilinykh PA, et al. 2018. PLoS Pathog. 14:e1007204. PubMed
  47. Spolski R et al. 2019. Elife. 8 pii: e45501. PubMed
  48. King LB et al. 2018. Cell host & microbe. 23(1):101-109 . PubMed
  49. Atyeo C, et al. 2022. Nat Commun. 13:3571. PubMed
  50. Boudreau CM, et al. 2022. Cell Rep. 38:110337. PubMed
  51. Atyeo C, et al. 2021. JCI Insight. 6:. PubMed
  52. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  53. Kaplonek P, et al. 2022. Immunity. 55:355. PubMed
  54. Bornholdt ZA et al. 2019. Cell host & microbe. 25(1):49-58 . PubMed
  55. Malherbe DC, et al. 2022. NPJ Vaccines. 7:83. PubMed
  56. Atyeo C, et al. 2021. Cell. 184:628. PubMed
RRID
AB_2563293 (BioLegend Cat. No. 305111)
AB_2563294 (BioLegend Cat. No. 305112)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD66b
Specificity Alt (DOES NOT SHOW ON TDS):
CD66b
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 3    Revision Date: 01/05/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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