- Clone
- P81B6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- V-Akt Murine Thymoma Viral Oncogene Homolog 2, Protein Kinase B Beta, Rac Protein Kinase Beta, RAC-PK-Beta, PKB Beta, PRKBB, PKBB, HIHGHH, PKBBETA, RAC-BETA
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P81B6_PURE_AKT2_Antibody_WB_092915 P81B6_PURE_AKT2_Antibody_WB_092915](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P81B6_PURE_AKT2_Antibody_WB_092915.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot analysis of cell lysates from HeLa (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3). Purified anti-AKT2 antibody (clone P81B6 ) was used and then was followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) was used as a loading control.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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680202 | 100 µg | $265 |
AKT (Protein kinase B, PKB) is a serine/threonine kinase that plays a key in regulating cell survival, insulin signaling, angiogenesis, and tumor formation. There are three isoforms of AKT- AKT1, 2, and 3, which are also known as PKBα, β, and γ). Increasing evidence suggests that AKT1, 2, and 3 plays an unique role in breast cancer initiation and progression. AKT2 is amplified or overexpressed in cancer with a higher frequency than those found with AKT1. AKT2 is also closely linked to Type II diabetes and is a pivotal player in the Phosphatidylinositol 3 kinase/Akt/Mammalian target of rapamycin (PI3K/mTOR) signaling axis. The deregulation of AKT2 has been associated with acquisition of stem cell-like properties, responsible for invasiveness and tumor progression, metastatic spread, and chemoresistance.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant fragment (a.a. 2-149) of human AKT2 expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2566344 (BioLegend Cat. No. 680202)
Antigen Details
- Structure
- 481 amino acids with a predicted molecular weight of approximately 56 kD.
- Distribution
-
Cytoplasm, nucleus, cell membrane, and peripheral membrane protein.
- Function
- AKT2 plays a ket role in regulating cell survival, insulin, signaling, angiogenesis, and tumor formation.
- Interaction
- BTBD10, KCTD20, MTCP1, TCL1A, TCL1B, CLK2, PBH2, TRAF6, and CLIP3.
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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1. Pereira L, et al. 2015. Drug Discov. Today 9:1152-8.
2. Yu H, et al. 2015. Vascul. Pharmacol. 71:57-64.
3. Cohen MM Jr. 2013. Am J. Med Genet A. 12:2931-7.
4. Dillon RL, Muller WJ. 2010. Cancer Res. 11:4260-4.
5. Song G, et al. 2005. J. Cell Mol. Med. 1:59-71. - Gene ID
- 208 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- AKT2
- Specificity Alt (DOES NOT SHOW ON TDS):
- AKT2
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about AKT2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All AKT2 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-AKT2 | P81B6 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-AKT2
Western blot analysis of cell lysates from HeLa (lane 1), Ju...
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