- Other Names
- GDNF family receptor alpha-1, GFRA1, Glial cell line-derived neurotrophic factor receptor alpha, GDNFR, GDNFRA
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Glial cell line derived neurotrophic factor (GDNF) family receptor alpha 1 (GFRα1) is a member of the GDNF receptor family. These receptors are glycophosphatidylinositol (GPI)‑anchored cell surface receptors containing 3 conserved cysteine repeats and mediate the activation of the RET tyrosine kinase receptor. GFRα expression is differentially regulated in the central and peripheral nervous system, indicating complementary roles for the GFRα members in mediating the activities of the GDNF family of neurotrophic factors. GFRα1 is a potent survival factor for central and peripheral neurons. GFRα1 mediates the GDNF-induced autophosphorylation and activation of the RET receptor tyrosine kinase. GDNF first binds GFRα1 and recruits RET, forming a signaling complex that is a pentamer containing one GDNF, two GFRα1s, and two RET molecules. The RET receptor tyrosine kinase represents the signal-transducing molecule of a multi-subunit surface receptor complex. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.Product Details
- Human GFRα1, amino acid Ala19-Lys429 (Accession # NM_145793.2), was expressed in CHO cells.
- Molecular Mass
- The 650 amino acid recombinant protein has a predicted molecular mass of approximately 72.6 kD. The DTT-reduced protein migrates at approximately 80 kD and and non-reduced protein migrates at approximately 160 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- When human GDNF is immobilized at 0.5 µg/mL, human GFRA1-Fc Chimera binds with EC50 of 10 - 40 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
Central and peripheral nervous system
- Receptor for the glial cell line-derived neurotrophic factor, GDNF
- Neural stem cells, Neurons
- Measured by its ability to bind human GDNF
- Cell Type
- Neural Stem Cells, Neurons
- Biology Area
- Neuroscience, Synaptic Biology
- Molecular Family
- Soluble Receptors
- Antigen References
- Baloh RH, et al. 1998. Proc. Natl. Acad. Sci. USA 95:5801-6.
- Baloh RH, et al. 1998. Neuron 21:1291-302.
- Nishino J, et al. 1999. Neuron 23:725-36.
- Honma Y, et al. 2002. Neuron 35:267-82.
- Veiga-Fernandes H, et al. 2007. Nature 446:547-51.
- Gene ID
- 2674 View all products for this Gene ID
- View information about GFRalpha1 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.