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Ultra-LEAF™ Purified anti-mouse CX3CR1 Recombinant Antibody

Pricing & Availability
Clone
QA16A03 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA16A03_PURE_CX3CR1_Recombinant_Antibody_070819.png
C57BL/6 mouse splenocytes were stained with CD11b APC and Ultra-LEAF™ Purified CX3CR1 (clone QA16A03) (left) or Purified Mouse IgG1, κ (right) followed by anti-mouse IgG1 PE.
  • QA16A03_PURE_CX3CR1_Recombinant_Antibody_070819.png
    C57BL/6 mouse splenocytes were stained with CD11b APC and Ultra-LEAF™ Purified CX3CR1 (clone QA16A03) (left) or Purified Mouse IgG1, κ (right) followed by anti-mouse IgG1 PE.
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Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse CX3CR1-transfected cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone SA011F11.

Product Citations
  1. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution

Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.

Function
Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References
  1. Hamon P, et al. 2017. Blood.129:1296
  2. Afik R, et al. 2016. J Exp Med. 213:2315
  3. Mass E, et al. 2016. Science. 353 pii: aaf4238
  4. Safaiyan S, et al. 2016. Nat Neurosci. 19:995
  5. Peng J, et al. 2016. Nat Commun. 7:12029
Gene ID
13051 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CX3CR1
Specificity Alt (DOES NOT SHOW ON TDS):
CX3CR1
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/13/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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