- Clone
- IRF4.3E4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon regulatory factor 4
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![IRF4.3E4_AF488_IRF4_Antibody_FC_022814 IRF4.3E4_AF488_IRF4_Antibody_FC_022814](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/IRF4.3E4_AF488_IRF4_Antibody_FC_022814.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF4 (clone IRF4.3E4) Alexa Fluor® 488 (open histogram with solid line: fresh cells, filled histogram: stimulated cells) or rat IgG1, κ Alexa Fluor® 488 isotype control (dash line: fresh cell, dot line: stimulated cells).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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646405 | 25 µg | $159 | ||||
646406 | 100 µg | $370 |
The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- GST fusion protein containing C-terminal of murine IRF4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2563266 (BioLegend Cat. No. 646405)
AB_2563267 (BioLegend Cat. No. 646406)
Antigen Details
- Structure
- Molecular weight is approximately 51 kD.
- Distribution
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IRF4 is reported to be expressed exclusively in the immune system.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Lu R. 2008. Trends Immunol 29:487.
- Gene ID
- 3662 View all products for this Gene ID
- UniProt
- View information about IRF4 on UniProt.org
Related FAQs
Other Formats
View All IRF4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 488 anti-IRF4 | IRF4.3E4 | ICFC |
Purified anti-IRF4 | IRF4.3E4 | WB,ICFC,ChIP |
PE anti-IRF4 | IRF4.3E4 | ICFC,SB |
Alexa Fluor® 647 anti-IRF4 | IRF4.3E4 | ICFC |
PE/Cyanine7 anti-IRF4 | IRF4.3E4 | ICFC |
PerCP/Cyanine5.5 anti-IRF4 | IRF4.3E4 | ICFC |
Alexa Fluor® 594 anti-IRF4 | IRF4.3E4 | ICC |
Pacific Blue™ anti-IRF4 | IRF4.3E4 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 488 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
Purified anti-IRF4
Total lysates (15 µg protein) from HeLa (lane 1, negative co... Chromatin Immunoprecipitation (ChIP) was performed using Go-... -
PE anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 647 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
PE/Cyanine7 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
PerCP/Cyanine5.5 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
Alexa Fluor® 594 anti-IRF4
A20 cells were fixed with 4% paraformaldehyde (PFA) for 10 m... -
Pacific Blue™ anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral...
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