- Clone
- SMI 71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Endothelial barrier antigen, EBA
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_SMI-71_A594_BBB_Antibody_1_032618 A_SMI-71_A594_BBB_Antibody_1_032618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SMI-71_A594_BBB_Antibody_1_032618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) and Alexa Fluor® 488 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibodies at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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836807 | 25 µg | $129 | ||||
836808 | 100 µg | $323 |
SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-P - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses -
Application References
(PubMed link indicates BioLegend citation) - RRID
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AB_2728552 (BioLegend Cat. No. 836807)
AB_2728553 (BioLegend Cat. No. 836808)
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Gene ID
- NA
- UniProt
- View information about Blood-Brain Barrier on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Blood-Brain Barrier Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Rat Blood-Brain Barrier | SMI 71 | IHC-P |
Biotin anti-Rat Blood-Brain Barrier | SMI 71 | IHC-P |
Alexa Fluor® 594 anti-Rat Blood-Brain Barrier | SMI 71 | IHC-P |
Alexa Fluor® 647 anti-Rat Blood-Brain Barrier | SMI 71 | IHC-P |
HRP anti-Rat Blood-Brain Barrier | SMI 71 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Rat Blood-Brain Barrier
IHC staining of purified anti-Rat Blood-Brain Barrier antibo... -
Biotin anti-Rat Blood-Brain Barrier
IHC staining of Biotin anti-Rat Blood-Brain Barrier antibody... IHC staining of Biotin anti-Rat Blood-Brain Barrier antibody... -
Alexa Fluor® 594 anti-Rat Blood-Brain Barrier
IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrie... IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrie... IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrie... -
Alexa Fluor® 647 anti-Rat Blood-Brain Barrier
IHC staining of Alexa Fluor® 647 anti-Rat Blood-Brain Barrie... IHC staining of Alexa Fluor® 647 anti-Rat Blood-Brain Barrie... -
HRP anti-Rat Blood-Brain Barrier
IHC staining of HRP anti-Rat Blood-Brain Barrier antibody (c...
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