- Clone
- S16015F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prominin-1, PROM1, AC133
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S16015F_A647_CD133_Antibody_071724 S16015F_A647_CD133_Antibody_071724](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S16015F_A647_CD133_Antibody_071724.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with anti-human CD34 (clone 561) PE and anti-human CD133 (clone S16015F) Alexa Fluor® 647 (left) or, mouse IgG2a, κ Alexa Fluor® 647 isotype control (right). Data shown were gated on the CD45+ CD14- lymphocyte population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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393913 | 25 tests | $171 | ||||
393914 | 100 tests | $375 |
CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD133 transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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In-house testing suggests that clone S16015F does not block clones 293C3, AC133, and clone 7 that are also raised against human CD133.
Antigen Details
- Structure
- 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains
- Distribution
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Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells
- Function
- May play a role in cell differentiation, proliferation, and apoptosis
- Interaction
- NAT8; NAT8B; CDHR1
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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- Yin AH, et al. 1997. Blood. 90:5002.
- Miraglia S, et al. 1997. Blood. 90:5013.
- Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
- Gene ID
- 8842 View all products for this Gene ID
- UniProt
- View information about CD133 on UniProt.org
Related FAQs
Other Formats
View All CD133 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD133 | S16015F | FC |
Purified anti-human CD133 | S16015F | FC |
APC anti-human CD133 | S16015F | FC |
PE/Dazzle™ 594 anti-human CD133 | S16015F | FC |
PE/Cyanine7 anti-human CD133 | S16015F | FC |
Brilliant Violet 421™ anti-human CD133 | S16015F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD133
NCCIT cells were stained with PE anti-human CD133 (clone S16... -
Purified anti-human CD133
NCCIT cells were stained with purified anti-human CD133 (clo... -
APC anti-human CD133
Human peripheral blood mononuclear cells were stained with C... -
PE/Dazzle™ 594 anti-human CD133
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human CD133
Human peripheral blood mononucleus cells were stained with C... -
Brilliant Violet 421™ anti-human CD133
Human peripheral blood mononuclear cells were stained with C...
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